Helichrysum Oil Inhibits IFN-γ and LPS Activity in RAW 264.7 Cells
Department
Biological Sciences
Advisor
Joy Doan
Document Type
Event
Version
Metadata Only
Abstract
The goal of this study is to examine the anti inflammatory properties of Helichrysum on macrophages from RAW 267.4 cells that will be stimulated by IFN-γ and Lipopolysaccharides (LPS) mediators into M1 phase inflammatory cytokines, and then analyze the results using a variety of analytical techniques, including morphology, phagocytosis assay, nitric oxide assay, and ELISA. Our hypothesis is that Helichrysum essential oil will inhibit the growth and change of macrophages stimulated by IFN-γ and LPS separately or combined, therefore limiting their inflammatory functions.
Recommended Citation
Everson, Leah; Reding, Caroline; Simmonds, Harrison; and Doan, Joy, "Helichrysum Oil Inhibits IFN-γ and LPS Activity in RAW 264.7 Cells" (2024). Science Symposium. 20.
https://spark.bethel.edu/science_symposium/spring2024/schedule2024/20
Helichrysum Oil Inhibits IFN-γ and LPS Activity in RAW 264.7 Cells
The goal of this study is to examine the anti inflammatory properties of Helichrysum on macrophages from RAW 267.4 cells that will be stimulated by IFN-γ and Lipopolysaccharides (LPS) mediators into M1 phase inflammatory cytokines, and then analyze the results using a variety of analytical techniques, including morphology, phagocytosis assay, nitric oxide assay, and ELISA. Our hypothesis is that Helichrysum essential oil will inhibit the growth and change of macrophages stimulated by IFN-γ and LPS separately or combined, therefore limiting their inflammatory functions.