Streptococcus gordonii SGO_0454 gene deletion in Biofilm Formation Under Fluid Shear
Department
Biological Sciences
Advisor
Brittany Nairn
Document Type
Poster
Version
Metadata Only
Abstract
Streptococcus gordonii is a Gram-positive bacterium that belongs to the Streptococcus family. It is commonly found in the oral cavity of the human mouth, but can also be found in the intestinal tract, and the skin. Biofilm formation is a complex and regulated process that enables different types of microorganisms to adhere to surfaces and form multicellular communities. S. gordonii is considered a commensal bacteria in the oral cavity that contributes to the multispecies biofilms known as dental plaque. Fluid flow has an important role in the transportation and dispersion of bacteria in the mouth allowing the formation of biofilms through adhesion and subsequent growth. SGO_0454 is a YebC/PmpR family regulator that encodes a predicted transcriptional regulator that was previously shown to be upregulated in biofilms. Here we investigated a deletion mutant of ΔSGO_0454 under fluid shear, it was evaluated under biofilm formation to see whether or not it was required under fluid flow when compared to the wild-type strain. Through means of stimulating saliva through individuals, which were pooled, centrifuged, and coated onto a 12-well plate. Biofilm formation assays utilized 12-well plates for negative control, wild type, and mutant of S. gordonii. The following bacterial inoculation, incubation, crystal violet staining, PBS washing, and treatment with ethanol were performed before measuring the absorbance. The deletion mutant, ΔSGO_0454, exhibits no significant impact in biofilm formation under fluid shear compared to the wild-type strain, this suggests that SGO_0454 plays an important role in the S. gordonii biofilm formation but not necessarily fluid flow or under this low amount of velocity.
Recommended Citation
Pratt, Hunter and Nairn, Brittany, "Streptococcus gordonii SGO_0454 gene deletion in Biofilm Formation Under Fluid Shear" (2024). Science Symposium. 30.
https://spark.bethel.edu/science_symposium/spring2024/schedule2024/30
Terms of Use and License Information
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Streptococcus gordonii SGO_0454 gene deletion in Biofilm Formation Under Fluid Shear
Streptococcus gordonii is a Gram-positive bacterium that belongs to the Streptococcus family. It is commonly found in the oral cavity of the human mouth, but can also be found in the intestinal tract, and the skin. Biofilm formation is a complex and regulated process that enables different types of microorganisms to adhere to surfaces and form multicellular communities. S. gordonii is considered a commensal bacteria in the oral cavity that contributes to the multispecies biofilms known as dental plaque. Fluid flow has an important role in the transportation and dispersion of bacteria in the mouth allowing the formation of biofilms through adhesion and subsequent growth. SGO_0454 is a YebC/PmpR family regulator that encodes a predicted transcriptional regulator that was previously shown to be upregulated in biofilms. Here we investigated a deletion mutant of ΔSGO_0454 under fluid shear, it was evaluated under biofilm formation to see whether or not it was required under fluid flow when compared to the wild-type strain. Through means of stimulating saliva through individuals, which were pooled, centrifuged, and coated onto a 12-well plate. Biofilm formation assays utilized 12-well plates for negative control, wild type, and mutant of S. gordonii. The following bacterial inoculation, incubation, crystal violet staining, PBS washing, and treatment with ethanol were performed before measuring the absorbance. The deletion mutant, ΔSGO_0454, exhibits no significant impact in biofilm formation under fluid shear compared to the wild-type strain, this suggests that SGO_0454 plays an important role in the S. gordonii biofilm formation but not necessarily fluid flow or under this low amount of velocity.