DNA replication is a form of template-directed polymerization that occurs with the aid of numerous enzymes. By developing a DNA replication mimic that can undergo template-directed polymerization without enzymes, the scope of template-directed polymerization will be broadened beyond biological systems. One template-directed polymerization method that has proven successful is reductive amination on a DNA template, a two-step polymerization reaction involving equilibrium imine formation followed by reduction of the imine to the amine. Because DNA-directed reductive amination is an information transfer system, it should proceed with fidelity; however, the extent of fidelity must be explored. These studies will require the ability to sequence the resulting ANP products formed under different reaction conditions Because traditional sequencing techniques for nucleic acids and polypeptides will not work to sequence ANP, we propose to study the fidelity of DNA-directed reductive amination by sequencing the ANP products using electrospray tandem mass spectrometry in the product ion scan mode, a methodology that has been used previously to sequence biopolymers including nucleic acids and polyamines.
Anderson, Sarah M., "Sequencing of Amine Nucleoside Polymers: Understanding the Fidelity of DNA-Directed Reductive Amination" (2011). Honors Student Works. 7.
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